Abstract
Study the resistance of bacterial and fungal isolates which identified previously towards different antibiotics that have been recommended for treating those patients. It was found that higher percentage of resistance to penicillin, gentamycin, tetracyclin, chlormphenicol, and trimethoprim- sulfmethazxol then moderate resistance to ampcillin, nalidixic acid, cephotaxime, streptomycin, cephalexin, rifampicin and less resistance to ciprofloxacin that represent the most effective antibiotic against uropathogenic bacteria. Ketazole antibiotic was used against yeasts, which the minimum inhibitory concentration of ketazole against 83% of Candida. albicans isolates was 128 μg/ml, the rest of the isolates was 64 μg/ml. Isolation of bacterial and yeasts DNA (chromosome + plasmids), results of DNA isolation showed that bacterial isolates (E.coli (RS1), proteus. mirabilis (RS2), klebsiella. pneumonia (RS3) and Pseudomonas. aeruginosa (RS4) have chromosome and plasmids vary in numbers and size according to isolate. While yeast (C13) isolate have only chromosome without plasmid.A result of curring shows that:-Resistance to ampicillin, tetracycline, gentamycin, pencillin for all bacterial isolates carried on plasmid.- Resistance to cephalexin by RS1 isolate, resistance to cephotaxim by RS3 isolate and resistance to chlormphnicol by RS4 isolate carried on plasmid, while resistance to other antibiotics were carried on chromosome.
Highlights
Urinary tract infection is an extremely common clinical problem and can be defined as the presence of microorgansims in a properly collected urine samples
Antibiotics are biochemical compounds naturally produced by certain types of microorganism that inhibit the growth or kill other microorganism [7]
Different types of antibiotics are discovered, some of them classified as broad spectrum antibiotic which effect on a wide range of bacteria (Gram positive and Gram negative), while others classified as narrow spectrum antibiotics effected on a limited type of microorganisms [8]
Summary
Urinary tract infection is an extremely common clinical problem and can be defined as the presence of microorgansims in a properly collected urine samples. Sensitivity of bacterial isolates were tested toward antibiotics (Ampicillin (AM), Cefotaxime (CTX), Cephalexin (CL), Chloramphenicol(C), Ciprofloxacin (CIP), Gentamicin (CN), Nalidixic acid (NA), Pencillin (P), Rifampicin (RA), Strteptomycin (S), Tetracycline (TE), Trimethoprim- Sulfmethaxazol (SXT)) by using the modified disc diffusion method. Sensitivity of Candida albicans isolates to antifungal agent and the determination of minimum inhibition concentration (MIC) [18]. After incubation period 48 hrs at 37 C, the result was recorded and compared with the control (SDB with Candida isolates) to determine the minimum inhibition concentration of the antifungal used. The isolates were highly resistant to Penicillin (P), Gentamicin (CN), Tetracycline (TE), Chloramphenicol (C) and Trimethoprim- Sulfmethaxazol (SXT) They were moderate in their resistance to Ampicillin (AM), Nalidixic acid (NA), Cephalexin (CL), Strteptomycin (S), Cefotaxime (CTX) and Rifampicin (RA), that were sensitive to each antibiotics, especially to Ciprofloxacin. All isolates were resistant to gentamicin and tetracycline which represent 90.47%, on other hand chloramphenicol and trimethoprim-Sulfmethaxazol which represent 80.51%; these results were in agreement with [25] who found that their isolates were resistant to the same antibiotics
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