Abstract
Pathogens present in the environment are the biggest source of diseases and epidemics in the breeding of laboratory animals. The presence of microorganisms, in fact, can critically influence the animal health status and consequently the validity and reproducibility of experimental data. In accordance with the 3Rs principle, this study fits into the Refinement and Reduction concepts. The development of a health surveillance plan on environmental material from animal housing would have an important impact not only on maintaining an adequate state of health and on the generation of quality experimental data, but also on reducing the number of animals to be sacrificed.
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