Detection of Pathogenic Escherichia coli (E. coli) Using Robust Silver and Gold Nanoparticles

Boken J

doi:10.4172/2157-7048.1000175

Abstract

The paper deals with differently synthesized robust metal nanoparticles using chemical synthesis approach for detection of a new pathogenic strain E. coli in water sample. The reactions have been completed using aqueous metal salt solution and trisodium citrate as a capping agent to obtain nanoparticles. The nanoparticles and their interaction against microorganisms have been characterized using UV-Vis spectroscopy and Transmission Electron Microscopy (TEM). For different concentration of sodium borohydride, surface plasmon resonance peaks have been obtained at 390 nm to 402 nm for silver nanoparticles and 518 nm to 524 nm for gold nanoparticles. It has been observed to be shifted from 520 nm to 527 nm and 395 nm to 408 nm for gold and silver nanoparticles after the interaction with E. coli respectively. Silver and gold nanoparticles have been synthesized at 80°C exhibited strong surface plasmon resonance peaks respectively at 433 nm and 529 nm. Remarkable shift have been noticed in surface plasma resonance peaks for small amount of E. coli added in the solution.

Highlights

Escherichia coli (E. coli) are members of a large group of bacterial germs that inhabit the intestinal microorganism of humans and other warm blooded animals
Silver and gold nanoparticles have been synthesized by wet chemical method using trisodium citrate as well as sodium borohydride at different reaction temperatures
Reduction using sodium borohydride is done at room temperature while trisodium citrate needs higher temperature ~80oC for the reduction

Summary

Introduction

Escherichia coli (E. coli) are members of a large group of bacterial germs that inhabit the intestinal microorganism of humans and other warm blooded animals. The presence of E. coli in water is strong indication of sewage or animal waste contamination and as few as 10 cells can cause serious human illness and even death. That’s why presence of E. coli in foodstuffs and drinking water is a chronic worldwide problem. It is estimated that due to bacterial cause thousands of food borne illness and hundreds of hospitalized and deaths each years. It is very important to monitor pathogenic E. coli strain and stop their growth by simple methods [3]. Human population growth and climate change are expected to increase in the number of species and concentrations of waterborne pathogens in surface water [5]

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Conclusion