Detection Of Panton–Valentine Leukocidin Gene In Methicillin Resistance Staphylococcus Aureus: Clinical And Epidemiological Aspects

Abstract

Introduction: The role of PVL in the disease process and severity of S.aureus till now is a debate matter. The data provide substantial evidence that the high virulence potential of CA-MRSA isolates is associated with the expression of PVL. PVL genes carried by HA-MRSA strains have also been recently described in cutaneous and invasive infections. Epidemiology of MRSA strains is constantly changing and is known to vary between hospitals. This study aimed to analyze the prevalence of PVL in MRSA isolates from patients using multiplex PCR. Material and Methods: Staphylococcus aureus were isolated from various clinical samples received in Bacteriology Laboratory using standard protocol. Antibiotic susceptibility testing was performed by Kirby – Bauer disc diffusion method. Detection of PVL genes by multiplex PCR.Results: The highest prevalence of MRSA PVL positive isolates was from pus sample (74.5%) followed by blood (16.3%). The results of antimicrobial susceptibility testing revealed almost similar pattern of antibiotics resistance among PVL negative MRSA isolates as compared to PVL positive MRSA isolates. Conclusion: The detection of PVL-producing MRSA will help us in framing and planning the infection control measures against the MRSA infections.