Abstract
Oral squamous cell carcinoma (OSCC) is the most common malignant tumor of the oral cavity and it account for 80-90% of all malignancies in the oral cavity. The aim of this study was to determine the presence of p53 mutations and to associate these mutations with the histopathological type of OSCC such as well differentiated and poorly differentiated. Analytical observational comparative study by cross sectional design was used. Forty untreated well and poorly differentiated OSCC biopsy sample and normal tissue biopsy material taken from 16 normal patients were analyzed for the presence of mutation in the conserved region of the p53 gene especially in exon 5 by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). The results of this study showed that p53 gene mutations were detected in exon 5; 11/40 (27.55%) with heterozygous mutation 9/11 (81.8%). The incidence in exon 5 of p53 gene mutation was significantly associated with well differentiated 2/20 (10%) and poorly differentiated 9/20 (45%) OSCPP (P=0,013). This study concludes that 1) mutation in exon 5 of p53 gene occurred frequently in OSCC; 2) exon 5 of the p53 gene could be one of the specific targets for histopathological grade of OSCC; 3) mutation in the exon 5 of p53 gene could be important prognostic factor in OSCC.
Highlights
Oralsquamoucsell carcinona( OSCC) $ the developmenptrocessespecialliyn earlyphaseofcell mostcommonmalignantumor of the oral cavjty, cycle Gl-S and lossof function p53 geneis the andits accounftor 80-90%ofall malignancieisn moslcommoncauseof OSCC.-Funhermorpe5. 1 oralcavity.Mortalityrat€remainshigh is about5% g€nehas been showna direct activationand an in worldwideand 2,4yo-3,Sini/no Indonesiaof all essentiarlol€to controlandto r€gulatecell cycleas cancers death.r' Until now, th€ molecular well as could induce apoprosis.aSubsequently, pathogenesoisfOSCCis still uncleaar ndcausedby inactivationof p53 genewould be the activityof the diagnosisis only basedon clinicopathological p53 disrupted results in uncontrolled cel examination.Consequ€ntly,the most fr€quent OSCC cases are found in advancedstage prolif€rationand if continuouslyoccur couldbe malignanciyn all €ancersa
Disruplion of the €ell cycl€ as well as humancancersi,t is noi clearwhethetrhep53 gene regulatorygenes compooentwhich involv€d in is involvedin OSCCsincefew studieshave controlingc€ll cycl€ is the main factor in the beenreportedprevioudyon p53 murationin OSCC
therol€of p5Jmuulion in theeliolog}ofoml cancerhasnol baenrigorouslystudi€din lndonesia PmruetvaiotiuosnstoufdiepsJhJav€geshnoewansasolocwiaetreidnwciidthencseeol'fl Jifferentiationiquamouscell carcinomain Japan How€ver, these studies have been based on an anatvsisof r€lativelyfew casesof oral squamous cell carcinoma5(oSCcl ln addition.association beMeenth€obsenedpan€mor incidenceof pJJ muBtions in OSCC wnh associated histopathological grade were not explored in s mbava. lndonesia. ln this sody' w€ examrned
Summary
Oralsquamoucsellcarcinorn(aOSCC) is themostcommonmalignantrumorofthe oralcavity,andils accountfor 80-90%ofall malignanciesin oral cavity. The aim of this studywasto detemine the pr€sence of p53 muiationsand to associatethesemutationswith the histopathologicaltype of OSCC such as w€ll differentiatedand poorly differentiated.Analitycal observationacl omparativestudy by crosssectionaldesign wasused.Forty untreatedwell and poorly difleren.iatedOSCCbiopsysampleand normalrissuebiopsy materialtakenfiom 16normalpatientswereanalyz€dfor thepresenceofmutation in the cons€rvedregion of thep gene€speciallyin exon by polymerascehainreaction-singlsetrandconformationpolymorphism (PCR-SSCPT).he resultsof this studyshowedthar p53 genemutarionsweredetectedin exon 5i ll/40 (27,5%)with heterozygoums utation9/ll (81,8%).The incidencein exon ofp genemutarionwas significantlyaccociatedwith well differentiated2/20 (l0o/o\ and poorly diferentiated9/20 (45%) OSCC(P=0,013T).hisstudyconcludetshat l) mutationin exon of p5l geneoccunedftequ€ntlyin OSCC; 2) exon of the p53 genecouldb€oneof the fte specifictargetsfor histopathologicgarl adeof OSCC; l)mutationin exon ofp genecouldbe importanpt rognosricfactorin OSCC.IndonesianJournatof Dentistn 2006: Edisi Khu:us KPPIKGXly:239242.
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