Abstract
Studies of the ability of the EPR spin trap DEPMPO to detect both superoxide and hydroxyl radicals produced by systems in vitro and in vivo are presented. Experiments using free radical-generating systems confirmed the suitability of the EPR spin trapping technique but also revealed the existence of an undesirable conversion of DEPMPO/OOH into DEPMPO/OH adducts. The rate of conversion decreases with oxygenation, and the production of oxygen-centered radicals increases. However, this property of DEPMPO does not have a significant influence on its ability to independently detect radicals produced by plant plasma membranes. Since the adduct conversion appears to be rather slow compared to radical generation, we conclude that the DEPMPO spin-trap can be efficiently used for detection of oxygen-centered radicals produced by systems in vivo, as demonstrated for isolated plasma membranes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
