Detection of oocysts of Cryptosporidium spp in gall bladder of sheep slaughtered in Mosul, Iraq

E G Suleiman,A A Al-Abadi,E T Butty

doi:10.33899/ijvs.2012.46812

Abstract

The current study revealed the presence of oocysts of Cryptosporidium in the fluid and tissue of gall bladder of sheep which is slaughtered in Mosul city by using different diagnostic techniques, including direct smear method, staining method by modified Zeil-Nelseen stain, scraping of mucosal layer of gall bladder and histological examination, the percentage of infection 20% and there were significant differences in the percentages of infection between gall bladder 20% and fecal samples 41%. The histological study showed that the oocyst of Cryptosporidium was attached on the surface of the epithelial cells of gall bladder. The high percentage of infection appeared in ewes was 30% and there was no significant differences in the infection of gall bladder between rams and ewes.

Highlights

‫‪The current study revealed the presence of oocysts of Cryptosporidium in the fluid and tissue of gall bladder of sheep‬‬ ‫‪which is slaughtered in Mosul city by using different diagnostic techniques, including direct smear method, staining method by‬‬ ‫‪modified Zeil-Nelseen stain, scraping of mucosal layer of gall bladder and histological examination, the percentage of‬‬ ‫‪infection 20% and there were significant differences in the percentages of infection between gall bladder 20% and fecal‬‬ ‫‪samples 41%
‫المختلفة وحدوث الاسھال المائي الغزير )‪ (٤‬تختلف دورة حياة‬ ‫ھذا الطفيلي عن دورة حياة الايميريا بكون مراحل الطفيلي‬ ‫التطورية تحدث ضمن مايعرف بالفجوة الطفيلية في منطقة‬ ‫الزغابات المعوية وتتبوغ أكياس بيضة الطفيلي داخل خلية‬ ‫المضيف وتصبح خمجة عند مرورھا مع البراز في حين أكياس‬ ‫بيضة الايميريا لاتتبوغ إلا بعد تركھا جسم المضيف )‪
‫الشكل ‪ :٢‬كيس البيضة لطفيلي الابواغ الخبيئة في القشطة‬ ‫المأخوذة من الطبقة المخاطية لكيس المرارة والمصبوغة بصبغة‬ ‫الزيل‪-‬نلسن المحورة الباردة باستخدام الكاميرا الرقمية بقوة تكبير‬

Summary

Introduction

‫‪The current study revealed the presence of oocysts of Cryptosporidium in the fluid and tissue of gall bladder of sheep‬‬ ‫‪which is slaughtered in Mosul city by using different diagnostic techniques, including direct smear method, staining method by‬‬ ‫‪modified Zeil-Nelseen stain, scraping of mucosal layer of gall bladder and histological examination, the percentage of‬‬ ‫‪infection 20% and there were significant differences in the percentages of infection between gall bladder 20% and fecal‬‬ ‫‪samples 41%.

Results

Conclusion