Abstract
Interphase fluorescence in situ hybridization (FISH) is a unique possibility to visualize nuclear DNA in all eukaryotic cells, whether dividing or not. This technology comprises a variety of FISH‐based approaches, which provide either qualitative or quantitative detection of DNA in cellular nuclei. Through hybridization to specific genomic loci or whole chromosomes, detection of nuclear DNA by interphase FISH has become an important technological basis for analyses of intercellular genome variability and molecular diagnosis. Furthermore, direct evaluation of structural DNA arrangement within the nucleus by interphase FISH gives an opportunity to uncover functional significance of genome and chromosome behavior at molecular and supramolecular levels throughout the cell cycle. This article reviews qualitative and quantitative detection of nuclear DNA using interphase FISH with special emphasis on technical details and key applications in biomedical research and analytical biochemistry.
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