Abstract
Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification.
Highlights
Tuberculosis (TB) is a disease caused by Mycobacterium spp
The decrease of resonance frequency was almost linear with the increase in concentration from 0.5 μM to 1.0 μM of thiol-modified oligonucleotide probe, and was stable for concentration 1.5 and 2.0 μM
The concentration of thiol-modified oligonucleotide probe at 1.5 μM was chosen for use in all experiments of this study
Summary
It is among the top ten causes of global mortality and morbidity, which had led to it becoming an important public health problem in Thailand. It is a slow-growing bacterium that needs 1-2 months for growing in culture [1,2]. Polymerase chain reaction (PCR) [5,6] is sensitive for detection of mycobacteria by using specific primers, but this amplification process requires additional processing time, reagents and devices, which affect the cost of assay. PCR analysis needs well-trained personnel [7,8]
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