Abstract
The prevalence of the recently identified astrovirus MLB1 in a cohort of children with diarrhea in St. Louis, Missouri, USA, was defined by reverse transcription-PCR. Of 254 stool specimens collected in 2008, 4 were positive for astrovirus MLB1. These results show that astrovirus MLB1 is circulating in North America.
Highlights
The prevalence of the recently identified astrovirus MLB1 in a cohort of children with diarrhea in St
The newly identified AstV-MLB1 virus was discovered in a stool specimen collected in Melbourne, Victoria, Australia, in 1999
We describe the detection of AstV-MLB1 in a cohort from St
Summary
The prevalence of the recently identified astrovirus MLB1 in a cohort of children with diarrhea in St. The Study Pediatric stool specimens sent for bacterial culture to the clinical microbiology laboratory at the St. Louis Children’s Hospital were analyzed for AstV-MLB1. Described astrovirus primers Mon269 and Mon270 [11] frequently have been used for detecting human astrovirus serotypes 1–8 in clinical stool specimens. Because AstVMLB1 might represent a new grouping of astroviruses that could include multiple subtypes, we designed primers to conserved regions of the AstV-MLB1 genome to maximize the likelihood of detecting any AstV-MLB1 variant viruses or even other novel astroviruses.
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