Detection of Newcastle Disease Virus Genome from the Field Outbreaks in Poultry by Reverse Transcription – Polymerase Chain Reaction

Abstract

A total of 30 field samples (27 tissue samples comprising of either trachea, lungs or brain; and 3 allantoic fluid samples) were collected from broiler chickens for detection of Newcastle disease virus (NDV) by RT-PCR. In addition, vaccine strains (F, LaSota, R B) were taken as positive control and trachea 2 from unvaccinated healthy bird as negative control. Newcastle disease virus (NDV) was detected in five field samples as well as in all the vaccine strains by RT-PCR. All these samples as well as vaccine strains yielded a band of 356 bp on amplification of F region of NDV. Three more field samples yielded a band of 216 bp with nested PCR, thus making total eight field samples positive for NDV. The results of the present study indicated that RT-PCR followed by nested PCR can be used to detect NDV directly from tissue samples in poultry.