Abstract
Poultry is one of animal protein sources that have been consumed much in Indonesia. Various of health disorders are caused by environmental changes, woof and infectious agents have caused decline in production and economic loss. One of diseases that have a big impact on poultry husbandry is Newcastle disease (ND). Newcastle disease virus (NDV) is an important disease in Indonesia, because this has spread around Indonesia and caused a big loss for poultry industry moreover, this has high morbidity and mortality and very rapid spread of the virus. The purpose of the research is to find out and determine a fast, exact and accurate, and efficient and effective application immunohistochemistry streptavidin biotin (IHK SB) test on NDV diagnosis affirmation and for NDV fast detection as a cause of disease on commercial laying hens with clinical torticollis and curled toe paralysis symptoms. The samples used in the form of tissues from 20 commercial laying hens with an indication of clinical torticollis and curled toe paralysis symptoms. Brains would be tested using streptavidin biotin. Inspection result data obtained by immunohistochemistry streptavidin biotin (IHK SB) method is analyzed in a descriptive and qualitative way. The result of the research proves that IHK SB coloration, NDV antigen could be detected on brains. Based on the result of the research, it could be concluded that IHK SB could be applied for NDV diagnosis affirmation and it proves that commercial laying hens with clinical torticollis and curled toe paralysis symptoms. Keywords: Newcastle disease virus, immunohistochemistry streptavidin biotin, torticollis, curled toe paralysis
Highlights
Newcastle disease (ND) is a deadly endemic disease caused by Newcastle disease virus (NDV), avian paramyxovirus type -1 (APMV-1)
Results of the present study showed that on the uninfected NDV tissues, the IHC SB staining was negative (Fig.1)
Chromogen conducted in IHC SB method is diaminobenzidine (DAB) that will give a result for the located antigen to change its color into reddish brown in the tissue preparation which is positively infected by NDV
Summary
Newcastle disease (ND) is a deadly endemic disease caused by Newcastle disease virus (NDV), avian paramyxovirus type -1 (APMV-1). NDV is considered as top ranking viral diseases of poultry by the Office International des Epizooties. These viral disease is responsible for serious economic losses to the poultry industry all over the world every year [1, 2]. ND is another highly contagious viral disease of many domestic and wild species of birds throughout the world [3] which is caused by an enveloped, single stranded, negative sense and nonsegmented genomic ribonucleic acid (ssRNA) virus. Velogenic Newcastle disease (VND) is the most severe form of the disease and is probably the most serious viral disease of poultry throughout the world. Newcastle disease was first reported in 1926 in the East Indies and in 1927 at Newcastle-on-Tyne, England, after which it is named
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