Abstract

A sensitive and rapid electrochemiluminescence (ECL) method for the detection of N 6-Methyladenosine (m6A) in urine samples on a heated indium-tin-oxide (ITO) electrode is presented. The ECL intensity of Tris(2,2′-bipyridyl) dichlororuthenium(II)hexahydrate (Ru(bpy) 3 2+) can be enhanced by the presence of m6A. Experimental results showed that the change of ECL intensities (Δ I) of the Ru(bpy) 3 2+ between before and after addition of m6A was affected by the working electrode surface temperature ( T e ); the highest Δ I occurred at 31 °C. Under optimum conditions, the Δ I had a linear relationship with the m6A concentration in the range of 1.9 × 10 −9–3.9 × 10 −6 mol/L and a detection limit of 7.7 × 10 −10 mol/L ( S/ N = 3) at T e = 31 °C. The recovery of m6A standards added to urine samples verified the accuracy of the proposed method.

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