Abstract
Over a one year period, 372 different cell cultures were received for the detection of mycoplasmas. PCR analysis of the 16SS rRNA gene was compared to DNA fluorescence staining by DAPI (4′,6-diamidine-2-phenylindole dihydrochloride). 278 samples (75%) were found to be negative using both methods, 86 samples (23%) were found positive with the PCR and the DAPI staining method, and 6 samples (2%) were found to be infected with other bacteria. The 86 positive samples were also subjected to specific amplification in order to identify the species. Only two of them (3.5%) could not be identified. In conclusion, PCR is a rapid and reliable method for detecting and identifying mycoplasmas in tissue cultures.
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