Abstract
Mycoplasma genitalum (MG) is associated with variety of urogenital infections such as nongonococcal urethritis (NGU), endometritis and cervicitis. The objective of this study was to demonstrate and evaluate a research polymerase chain reaction (PCR) assay, for the detection of MG in cervical samples of a tested population of women attending gynecology clinics in Bosnia and Herzegovina. The Multitarget Real-Time (MTRT) PCR, utilizing the ABI 7900HT, the sequence detection system, was performed for the detection of MG. Cervical samples (N=97) from females were divided into three types of patient groups: Group 1: patients who had known abnormal clinical cytology reports (N=34); Group 2: patients who reported a history of genitourinary infections (N=22); and Group 3: patients not in either groups 1 or 2 (N=41). Overall, 14,43% (14/97) of those tested were positive for MG. A positive sample was defined as having a cycle threshold cross point (Ct) < 40,0 with a fluorescent detection comparable to the low positive control utilized during the run. This study validated the use of MTRT PCR as a reliable method for the detection of MG in clinical specimens and should facilitate large-scale screening for this organism.
Highlights
Mycoplasma genitalum (MG) is associated with variety of urogenital infections such as nongonococcal urethritis –NGU ( - of cases), endometritis and cervicitis ( )
For the whole population, ( / ) of the population were MG positive by Multitarget Real-Time (MTRT) polymerase chain reaction (PCR) (Table )
The MTRT PCR did limit the number of potential false positive results during the study with high-throughput detection of Mycoplasma genitalium because two gene targets were evaluated
Summary
Mycoplasma genitalum (MG) is associated with variety of urogenital infections such as nongonococcal urethritis –NGU ( - of cases), endometritis and cervicitis ( ). Mycoplasmas are the smallest free-living prokaryotic microorganisms They posess unique characteristics among prokaryotes such as the lack of cell walls and complete insensitivity to the penicillins and cephalosporines. These organisms have sizes of about , - , μm, but they are highly plastic and pleomorphic and may appear as coccoid bodies, filaments, and large multinucleoid forms. Laboratory detection includes culture and molecular techniques. Different polymerase chain reaction (PCR) assays for detection of MG have been developed, but there are some limitations including the potential for false positive results, with PCR techniques, as well as false negative results when using culture. The multi-target real-time (MTRT) PCR for MG utilizes two different gene targets; the MgPa gene and S ribosomal gene and was developed to limit the number of false positives generated ( )
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