Detection of Mycoplasma gallisepticum and Mycoplasma synoviae by using of cultural and PCR technique

N.A Jafar,Bashar Noomi

doi:10.33899/ijvs.2019.125484.1016

Abstract

Laboratory methods are essential for the diagnosis of Mycoplasmal infection. There are three laboratory approaches are essential for the diagnosis of Mycoplasmal infection in chicken including direct methods by culture method and polymerase chain reaction, and indirect methods by detection of Mycoplasmal antibodies by serological tests. This study aimed to detection of Mycoplasma by culture and PCR technique. Two hundred seventy-six samples were collected from infected adult boiler chicken in Salah Al-din province which suffering from respiratory signs and /or joint infection, 202 respiratory and 74 articular samples. According to the results of culture, Mycoplasma isolated in rate of 35.1% (36.6% from respiratory samples and 31.1% from articular samples). The sensitivity of culture was 100%, while the specificity of culture was 97.9% when comparing with PCR results. The current study concluded that the respiratory infection was more than articular infections, and Mycoplasma gallisepticum more distributed than Mycoplasma synoviae among chickens.

Highlights

Avian Mycoplasmosis are highly contagious disease of poultry
There are more than 120 species, avian mycoplasmosis mainly caused by Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS)
Classification of Mycoplasma isolates into MG and MS The Diagnosis of isolated Mycoplasma into species was done according to the DNA content of the isolated microorganisms by using of PCR

Summary

Introduction

Avian Mycoplasmosis are highly contagious disease of poultry. The disease called chronic respiratory disease in chicken and infectious sinusitis in turkey [3,4]. It causes a highly economic loss by decreasing in hatching rate, exclude of infected Chicks, decrease in eggs production, reduced growth rate, increased costs of eradication procedures which involve site cleaning and depopulation, and increased costs of medication and vaccination [5,6]. Laboratory methods are essential for the diagnosis of Mycoplasmal infection, since, clinical signs and pathological lesions cannot reliably diagnose the source of infection. Rapid and early diagnostic detection of Mycoplasmal infections is important to prevent the spread of infection and to limit economic losses in the poultry industry. There are three approaches to diagnose mycoplasma infection: isolation and identification of organism by culture techniques, detection of its DNA, and identification of specific antibodies by serological methods like ELISA, serum plate agglutination test, and hemagglutination inhibition test [10,11,12]

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