Abstract
Detection of Mycobacterium avium subsp. paratuberculosis from cattle and buffaloes in Egypt using traditional culture, serological and molecular based methods
Highlights
Aim: This study aimed to evaluate three conventional diagnostic methods for Mycobacterium avium subsp. paratuberculosis (MAP) under the Egyptian circumstances with a general aim to determine the appropriate strategy to develop a Johne's disease (JD) control program
Out of 50 pooled specimens, 34 (68%) fecal cultures were positive for MAP
Johne's disease (JD) is one of the most wide-spread and economically important disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP) [1]
Summary
Johne's disease (JD) is one of the most wide-spread and economically important disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP) [1]. Johne's disease (JD) is one of the most wide-spread and economically important disease of ruminants caused by Mycobacterium avium subsp. The relatively long incubation period is characterized by the excretion of MAP in feces for months and years before clinical symptoms develop. The exposure to contaminated feces constitutes one of the main risk factors for MAP transmission within the herd [4]. The definition of an infected animal based either on the presence of anti-MAP antibodies in the serum, or bacterial culture from tissue or feces [6]. Johne's disease (JD) caused by Mycobacterium avium subsp. The definition of an infected animal based either on the presence of anti-MAP antibodies, or positive bacterial culture. Some primary non intensive studies confirm presence of JD in Egypt, the disease is currently neglected by the official Egyptian veterinary agencies. There is no official data, no national control program, and no used vaccine
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