Detection of mutation in Exon 2-3 in Perforin gene and Exon4 in Fas gene in sample leukemia Iraqi patients

Abstract

This work aimed to study the possible mutations in Perforin gene (PRF1) exon 2-3 and Fasgene exon 4 in Acute Lymphocytic Leukemia (ALL) and Chronic Lymphocytic Leukemia(CLL) patients from Iraq. In an attempt to detect any mutation within PRF1 gene and Fas genes,a sequencing analysis for these genes were made. The results were alignment with sequencespresent in the Gene Bank seeking for homology and differences. A DNA sequence for Homosapiens PRF1 gene was found compatible with genes of ALL, CLL patients and healthycontrols, 100% compatibility was found in the flank DNA sense and antisense sequences fromhealthy. However, 99% compatibility was detected for the genes isolated from ALL patientswith an insertion of C697 and A698 G in the flank DNA sense strand and insertion of G697 andT698C in flank DNA antisense strand of the gene. Morever, 99% compatibility was detected forthe genes isolated from CLL patients with two transition mutations in the flank DNA sensestrand of C957T and C1035T and one transition mutation in the flank DNA antisense strand ofG957A. However, no mutations were detected in Fas gene isolated from ALL, CLL, and healthycontrols.