Detection of mutagens and BaPMO inducers in river water using the Blue Cotton adsorption technique

Abstract

Induction of Mixed Function Oxidase (MFO) activity and bioactivation potential were measured in experimental carp and in native fish species from two rivers with different pollution level (Sava and Mrežnica). The experimental carp were intraperitoneally exposed to various water volume equivalents of Blue Cotton extracts from both rivers. The induction of MFO measured as a Benzo(a)pyrene monooxygenase (BaPMO) activity was increased up to 9.3-fold in experimental carp and up to 11.3-fold in native fish from Sava River, whereas the values from Mrežnica River showed only a slight increase when compared with the control (highest increase of 1.8-fold in nose carp). Accordingly, bioactivation potential using modified Ames test was higher in both experiments with Sava River. Both measured parameters in experimental carp increased in a dose-dependent manner in accordance to river volume equivalents. Different induction potential of native fish species comparable between two rivers confirmed the known possibility of their usage in biomonitoring studies. These results give qualitatively a new support to the idea of using Blue Cotton extraction technique combined with induction of MFO activity and Ames test in fish as a good biomarker for assessing risk of exposure to mutagens/carcinogens such as Polycyclic Aromatic Hydrocarbons (PAHs), especially due to the simplicity and cost-effectiveness of these methods.