Abstract
Peritoneal dialysis (PD) is an underutilized mode of dialysis therapy worldwide. Despite the reduction in peritonitis rate, peritonitis continues to be the main cause of technique failure. This study was undertaken to detection of the multiple bacterial strains in dialysis patients by melting curve analysis Real-time (RT-PCR). Ninety specimens of dialysate collected were blood culture and primers RT-PCR detection of the pathogens (bacterial). The results showed the most detected bacteria were Streptococcus pneumoniae (33%) followed by E. coli (31%), Staphylococcus aureus (13%), Klebsiella pneumoniae 11% then Pseudomonas aeruginosa (7%) respectively while the least frequency were recorded with Enterococcus faecalis with only (5%). From this study can concluded the identification of pathogens within two hours, Six bacterial pathogens have been detected and differentiated by using multiplex RT-PCR with peaks for melting curve study, high repeatability was exhibited for every amplification product.
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