Abstract
Detection of multiple autoantibodies in patients with ankylosing spondylitis using nucleic acid programmable protein arrays
Highlights
From the ‡Human Immunology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, United Kingdom, the **Harvard Institute of Proteomics, Harvard Medical School, Cambridge, Massachusetts 02141, the §Central Proteomics Facility, Henry Wellcome Building for Molecular Physiology, Department of Clinical Medicine, University of Oxford, Oxford OX3 7BN, United Kingdom, the ‡‡Virginia G
From the screening of nucleic acid programmable protein array (NAPPA) array GST#1, 193 autoantigens were shared by three Ankylosing spondylitis (AS) patients, 82 autoantigens were shared by four AS patients, 30 autoantigens were shared by five AS patients, and three autoantigens were shared by six AS patients (Fig. 4A)
We have used a novel type of protein array screening tool to characterize the autoantibody response in patients with ankylosing spondylitis
Summary
The extremely strong association with the Class I human leukocyte antigen allotype HLA-B27 has led to hypotheses involving CD8 T cell-mediated immunity [3]. Autoantibodies to cyclic citrullinated peptides have proven more specific than rheumatoid factor in diagnosing RA and have been shown to have prognostic value [7]. Increased levels of circulating plasma cells have been reported in AS patients [11], as well as evidence of hypergammaglobulinemia [12]. Aside from these findings, no comprehensive investigation into the presence of autoantibodies in patients with AS has been performed to date.
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