Detection of multidrug-resistance Klebsiella pneumoniae isolated from oropharyngeal swab in broiler chickens

Abstract

Klebsiella pneumoniae is an important pathogen that causes infection in the respiratory system of animals and humans. Livestock and poultry therapy for bacterial infections, such as K. pneumoniae, further increases the risk of microbial resistance to antibiotics and impacts human health globally. This study aimed to detect multidrug-resistant K. pneumoniae in the oropharyngeal swab of broiler chickens. The Samples were collected from 100 broilers in closed-house and open-house system cages (50 samples of each). Identification conducted by fenotipic and genotypic characterization. Isolates characterized are followed by antibiotic sensitivity testing using Gentamicin, Ciprofloxacin, Chloramphenicol, Erythromycin, Clindamycin, and Tetracycline. The study successfully identified 5 Klebsiella pneumoniae isolates. Two isolates (4%) were isolated from closed-house system cages, and 3 isolates (6%) were isolated from open-house system cages. Two isolates from closed-house system cages have shown resistance to tested antibiotics except Chloramphenicol, while the 3 isolates from open-house farms still showed sensitivity to Ciprofloxacin (66%) and Chloramphenicol (33%). According to the results, it can be concluded that multidrug-resistant Klebsiella pneumoniae can be isolated from closed-house and open-housed system cages with different prevalence rates.