Abstract
：Objective To evaluateefficiency of plague component vaccines by detection of mouse serum antibody titer andobservation of protecting guinea pigs against virulent Yersinia pestis challenge.MethodsThe plague component vaccines were prepared by admixing 15μg F1 antigen,15 μg rVantigen and 15μF1+15μg rV antigen with aluminumhydroxide adjuvant,respectively.NIH mice and guinea pigs were immunized with 2 doses ofplague component vaccines subcutaneously at a schedule of 0 and 2 weeks.The animals wereimmunized with one dose of live attenuated plague vaccine for percutaneeus scarificationas a control group.Serum anti-F1 and anti-rV IgG titers in mice were determined byindirect ELISA 6 weeks after immunization.and guinea pigs were subcutaneously challengedwith virulent Y.pestis strain 141 to observe the efficiency of the phgue componentvaccines.t test was adopted in the comparison between the groups.Results The results ofantibody determination showed that anti-F1 IgG or/and anti-rV IsG levels in three plaguecomponent vaccine groups were much higher than those in live attenuated plague vaccinegroup,and these were statistically significant differences between plague componentvaccine groups and live vaccine group(t=3.041,3.472,15.958,16.264,P＜0.01).The results of challengetest in guinea pigs showed that the survival rate in F1+rV vaccine group was 90%(9/10),andthose in F1 vaccine group and rV vaccine group were 50%(5/10)and20%(2/10),respectively.Conclusions The plague F1+rV vaccine is effective for preventingplague,and F1 vaccine or rV vaccine is unable to provide effective protection againstvirulent Y.pestis challenge. Key words: Yersinia pestis; Phsue vaccine; Serum antibody
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