Detection of mouse and rat urinary aeroallergens with an improved ELISA

Abstract

BackgroundRisk analysis of laboratory animal work presupposes allergen monitoring with sensitive methods. Commercial ELISA kits have recently become available for the detection of mouse (Mus m 1) and rat (Rat n 1) urinary allergen from settled dust samples and air samples with high allergen levels. ObjectiveOur aims were to enhance the sensitivities of the commercial ELISA kits for low aeroallergen levels (less than 1 ng/m3) and to test these methods with air samples collected from an animal facility. MethodsPersonal and stationary air samples were collected from an animal facility during various tasks of laboratory animal work and from various premises of the animal facility. ResultsThe sensitivities of the ELISA assays were improved with a careful choice of analysis parameters and reagents. The detection limits of 0.1 ng/m3 for Mus m 1 and 0.8 ng/m3 for Rat n 1 were established. The sensitized assays enabled detection of mouse and rat aeroallergens also from premises in which animals or dirty cages were not present during sampling. ConclusionThese sensitive assays will help to perform risk assessment in laboratory animal work. However, there remains a lack of standardized analytic procedures and occupational exposure limits for laboratory animal allergens.