Abstract
ABSTRACTThe mold colony count method was compared with the mold‐ELISA for enumeration of molds in nuts and spices. Mold counts were determined using three different enumeration media (dichloran‐rose bengal medium, dichloran‐glycerol medium and malt extract medium). The mold‐ELISA was carried out with antibodies raised against the heat stable, water‐soluble polysacharide antigens produced by molds. It was demonstrated that the reliability of the mold‐ELISA could be enhanced by specific inhibition. For this inhibition specific sugar derivates, which neutralized the antibody activity, were added to presumptive positive test samples resulting in a negative ELISA reaction. It became clear that the mold colony count is a poor reflection of the real state of mold presence in nuts and spices.
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