Abstract
We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target.
Highlights
We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay
The speci city of Watson–Crick base pairing associated with sequence-directed hybridisation of nucleic acids provides a high level of control over the self-assembly of nanostructures, and this unique mechanism has inspired the use of DNA in bioengineering and biosensing applications
We recently demonstrated that DNA-mediated liposome–liposome fusion could be triggered by the speci c interaction with a target microRNA, and that this mechanism could serve as the basis of a miRNA detection assay.[10]
Summary
We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target.
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