Detection of methylation status of Caveolin-2 gene in gastric carcinoma

Abstract

AIM:To investigate the relationship between methylation of 5'CpG island in the promoter region of Caveolin-2 gene and development of gastric carcinoma. METHODS:Using methylation-specific PCR technique,the methylation status of the 5'CpG island in the promoter region of Caveolin-2 gene was measured in 33 tissue samples of gastric carcinoma and 5 normal gastric tissue samples 5 cm apart from tumor. RESULTS:Methylation of the 5'CpG island in the promoter region of Caveolin-2 gene in all 5 samples of normal gastric tissues was negative.In 33 gastric cancerous tissue samples,the methylation positive rate of promoter region of Caveolin-2 gene was 87.9% (29/33),among which 20 cases (60.6%) complete methylation,9 cases (27.3%) were partial methylation,and 4 samples were negative. Statistics showed the methylation rate of the promoter region of Caveolin-2 gene was obviously higher in gastric carcinoma than in normal gastric tissues. CONCLUSION:The methylation positive rate of the 5'CpG island in the promoter region of Caveolin-2 gene in gastric carcinoma is high,indicating that its methylation may be related to genesis of gastric carcinoma.