Detection of metallo-β-lactamase gene blaIMP of Escherichia coli clinical isolates in Sanglah General Hospital Bali

Abstract

Background: Escherichia coli belong to the family of Enterobacteriaceae that are responsible as one of the leading cause of nosocomial infections. The emergence of Carbapenem-resistant Enterobacteriaceae presents therapeutic challenges for clinicians on choosing the correct type of antibiotics to prescribe. The Emergence of microorganism capable of producing metallo-β-Lactamase (MBL) causes resistance to several antibiotic, including carbapenem. Aim: The study aims is to detect whether there are E.coli clinical isolates in Sanglah hospital that produce metallo-β-Lactamase (MBL) enzyme and carries blaIMP metallo-β-Lactamase (MBL) gene.Methods: Clinical isolates of E.coli were collected from patients diagnosed with infection in Sanglah Hospital within January to June of 2018 nd has been subjected to antibiotics susceptibility test using Vitek-2 Compact system. EDTA-DDST was performed to detect MBL producing strains, followed by detection of blaIMP gene using PCR.Results: There were no E.coli clinical isolates that produce MBL enzyme and carried blaIMP gene (0.0%). The prevalence of E.coli producing blaIMP gene in Sanglah hospital was 0.0%.  Conclusions: None of the Escherichia coli exhibits the ability to produce metallo-β-Lactamase (MBL) enzyme and carried blaIMP gene. Detecting other genes aside from blaIMP gene may be necessary to reveal the other causative factors of antibiotics resistance in Escherichia coli.Â