Abstract
This unit provides a discussion of high-pressure liquid chromatography-atmospheric pressure ionization mass spectrometry (LC-API-MS) for separation, detection, and identification of products from xenobiotic metabolism. It includes an introduction and important technical information for electrospray and atmospheric pressure chemical ionization methods as well as scan modes. Emphasis has been placed on each method as related to phase I and phase II metabolite identification and there are listings of specific applications (and references) to reduce method development time.
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