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Abstract
A total of 60 random samples of meat were collected from different butchers shops at Port- Said city and subjected to bacteriological examination. The prevalence of Staph. aureus coagulase positive was 10 (16. 6%). The coagulase positiveStaph. aureus isolates were tested for their susceptibility to methicillin/ Oxaciillin. 6(60%) of the isolates were resistant to methicillin/ Oxaciillin. Multidrug resistance of MRSA strains was observed. The maximum resistance was observed against Ampicillin and Chloramphenicol (100%) while the least resistance was observed against Streptomycin (66.6%) followed by Gentamicin and Trimethoprim + Sulphamethoxazole (33.3%) and Erythromycin (16.6%). The isolates were analyzed for the presence of the genes encoding resistance to methicillin (mecA) by using Polymerase chain reaction (PCR) which is a powerful and a rapid procedure for the detection of mecA gene in methicllin-resistance (MRSA) strains. Only 3 out of 6 isolates were found to carry the mecA gene amplified at 310 bp. The Staph. aureus found in meat directly affect the human health and can cause a public illness if the meat is used in the food industry. Examination of meat using convetional PCR is a useful technique for detection of mecA gene inMethicillin-resistanceStaph. aureus (MRSA).
Highlights
Staph. aureus is the most commonly isolated human bacterial pathogen and is an important cause of skin and soft tissue infections, endovascular infections, pneumonia, septic arthritis, endocarditis, osteomyelitis, foreign-body infections, and sepsis (McDougal et al, 2003)
The first family is known as the community-acquired Methicillin-resistant Staph. aureus (MRSA) (CAMRSA) strains and are associated with strains acquired in community settings and are expected to be sensitive for many antibiotics
The Polymerase chain reaction (PCR) assay confirmed the presence of mecA gene in 3strains (50%) at 310 bp Table (5) and Figures (1), which is agree with (Riffon et al, 2001; John, 2003; Sajith Khan et al, 2012 and Kamal et al, 2013) who found that PCR assay was rapid and accurate procedure for the detection of MRSA strains as compared to the conventional methods since the reporting time is less and can help efficiently in infection management
Summary
Staph. aureus is the most commonly isolated human bacterial pathogen and is an important cause of skin and soft tissue infections, endovascular infections, pneumonia, septic arthritis, endocarditis, osteomyelitis, foreign-body infections, and sepsis (McDougal et al, 2003). Aureus is the most commonly isolated human bacterial pathogen and is an important cause of skin and soft tissue infections, endovascular infections, pneumonia, septic arthritis, endocarditis, osteomyelitis, foreign-body infections, and sepsis (McDougal et al, 2003). Aureus (MRSA) is considered one of the most common pathogens causing nosocomial infection (Shore and Coleman, 2013). MRSA strains are categorized into two families. The first family is known as the community-acquired MRSA (CAMRSA) strains and are associated with strains acquired in community settings and are expected to be sensitive for many antibiotics. The second family is the hospital-acquired MRSA strains which originate from hospital infections and are resistant to many antibiotics. Foodstuffs have been considered as sources of MRSA strains, surveying and surveillance activities for foodstuffs harboring MRSA strains is needed (Yamamoto et al, 2013)
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