Abstract
This study investigated the potential effects of Malathion in rabbit carcasses on the developmental of Chrysomya megacephala larvea, an important forensic species, and their possible implications for the calculate the postmortm interval ( PMI) .Three domestic rabbits Oryctolagus cuniculus were used in each experiment. Two rabbits were administered orally with 513 mg/kg (R1), 1026 mg/kg (R2) of Malathion, The third one was fed with distilled water (R0).Chrysomya megacephala larvae were allowed to grow on the liver (L), muscles (M) and all carcasses of rabbit. Malathion was detected in all collected rabbit tissues that received different dosages of Malathion (R1 and R2) but not detected in any of the controls (R0).The highest Malathion concentration was detected in lungs followed by muscle tissue and fats of R2(35.7 mg/kg, 29.75 mg/kg, and 22.31mg/kg, respectively). All third larval instars and pupae of C. megacephala were positive for malathion (R1 and R2), while malathion was not detected in all samples from the control colony (R0). Strong correlations were found between administered dosage and tissue concentrations. Malathion concentrations were higher in the third larval instars sampled for the concentration (R2) than those from muscle tissues of (R2) which followed by those from L2 colonies. Detected Malathion concentrations in the third larval instars and pupae of C. megacephala were significantly lower than those detected in the rabbit tissues except fats and heart from postmortem interval rabbit tissues treated with (R1). Moreover, the highest levels of Malathion were noticed in the pupae emerged from (R2) colony. Whereas, the lowest levels were detected in the pupae emerged from M1 colony.
Highlights
Blowflies provide valuable clues in many aspects of legal investigations, in estimating the time of death in cases where the postmortem interval (PMI) is prolonged and the value of other methods is limited (Campobasso and Introna, 2001)
Our study determines the levels of Malathion residues in rabbit tissues and immature stages of Chrysomya megacephala feeding on rabbit's carcasses treated with Malathion
Detection of Malathion in Rabbit Tissues: Results in Table (1) showed that Malathion was detected in all collected rabbit tissues that received different dosages of Malathion (R1 and R2) but not detected in any of the controls (R0)
Summary
Blowflies provide valuable clues in many aspects of legal investigations, in estimating the time of death in cases where the postmortem interval (PMI) is prolonged and the value of other methods is limited (Campobasso and Introna, 2001). Some death that occurs by poisoning remains undiscovered until the body is wholly or partially skeletonized. In such cases, the analysis of toxicology using body fluids and tissues is almost impossible. Fly larvae (maggots) involved in processing the corpse tissues would likely ingest any chemical metabolites from the corpse into their own tissues. These insects can be analyzed to detect those substances. Fly larvae from decomposing bodies can serve in the estimation of postmortem interval and can be used in qualitative identification of drugs or toxins (Rodriguez et al 1993 and Gunatilake& Goff (1989). Our study determines the levels of Malathion residues in rabbit tissues and immature stages of Chrysomya megacephala feeding on rabbit's carcasses treated with Malathion
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