Detection of lysozyme adsorption on titania using surface plasmon resonance on Au layer coated with thin titania film

Abstract

Titanium metal, which is used as artificial bone, has a titania surface layer. In this study, titania was investigated using surface plasmon resonance (SPR) in terms of protein adsorption, which occurs during the initial process of cellular attachment by employing a multilayer device consisting of a titania layer on Au. The titania film was deposited on the Au layer in the multilayer device by atmospheric chemical vapor deposition. A flow cell was used to evaluate protein adsorption on the titania top layer of the multilayer device. Phosphor buffer solutions with and without the protein lysozyme (Lyz) were injected into the flow cell. From the attenuation of reflected light, the SPR angle was determined as the angle with minimum reflection intensity. The observed behavior of the SPR angle indicated that Lyz was adsorbed on the titania film surface, and the obtained SPR angles increased with the Lyz concentration on the titania surface. These results indicate that the titania/Au multilayer SPR device can easily be used to detect the adsorption of protein.