Abstract
In this paper, a graphene-coated surface plasmon resonance sensor is designed for the examination of Rodent urine which is responsible for Leptospirosis bacteria. Rodent urine is considered as sensing medium. Graphene surface is activated by phosphate-buffered saline solution for better attachment of Leptospirosis bacteria on its surface. Oliguria and Polyuria are the Rodent urine with high and low concentrations of Leptospirosis bacteria, respectively. The transfer matrix method is used for the formulation of reflection intensity of p-polarized light. The reflectance curves for angular interrogation are plotted and the results are obtained in terms of sensitivity, detection accuracy, and quality factor. The significantly high sensitivity and detection accuracy for Oliguria distinguishes it from Polyuria having lower sensitivity.
Highlights
Leptospirosis is widely spread zoonosis in the world
Leptospirosis bacteria presented in Rodent urine can be immobilized on the graphene surface by the enhanced attachment through phosphate buffered saline (PBS) solution
The change in refractive index (RI) will affect the propagation constant of surface plasmons waves resulting in a disturbance in the resonance condition, which can be further achieved at different angles of incidence
Summary
Leptospirosis is widely spread zoonosis in the world. Leptospirosis caused by spirochetes belongs to Leptospira genus which is sub-classified in pathogenic and saprophytic species (chemoheterotrophic in nature). The surface plasmon resonance (SPR) sensor is a label-free detection technique in real-time. The SPR sensor has capability to detect the presence of single biomolecule with high sensitivity. As far as the performance of the SPR sensor is concerned, silver has moderate sensitivity with the highest detection accuracy and quality factor, and copper has the highest sensitivity [10]. There should be some material which has higher affinity towards plasmonic metal as well as antibody/antigen for Leptospirosis. It is believed that Leptospirosis bacteria can be adsorbed on the graphene surface. Surjeet RAIKWAR et al.: Detection of Leptospirosis Bacteria in Rodent Urine by Surface Plasmon Resonance
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