Abstract
Background: Legionellae can be isolated from various sources of water. Legionella pneumophila istransmitted via contaminated water and caused many diseases like pneumonia; lungs abscesses and fever.Objectives: Isolation and identification of L. pneumophila from environmental samples; study the factorsthat affect the frequency of L. pneumophila; and some oxidizing substances on their growth.Materials and Method: A total of one hundred water samples were collected from Cooling tower; Watertanks; Tap water and Swab from inner tap water. All samples were cultured on Buffered charcoal yeast extract(BCYE) agar and Buffered charcoal yeast extract (BCYE) broth to isolate Legionella pneumophila andLegionella dumoffii. Effect of pH; temperature; chlorine; Iron and effect of soluble zinc on L. pneumophilagrowth were studied.Results: The isolates gave positive results for the tests of catalase, oxidase, gelatin degradation, nitratereduction and beta-lactamase. Iron; Soluble Zinc; Chloride; Calcium hardness (CaCO3); Turbidity and silverwere ? 3ppmas; 1-3 ppmas; 0.2-0.5 ppm; 600 ppm; ? 0.3 and 3 ppmas respectively. The distribution of L.pneumophila in the water samples were studied.Conclusion: FatalL. pneumophila may be transmitted by drinking contaminated water and led to deathbecause they caused pneumonia.
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