Abstract

The evaluation capability of hyperspectral imaging technology was studied for the forecasts of heavy metal lead concentration of oilseed rape plant. In addition, a transfer stacked auto-encoder (T-SAE) algorithm including two network methods, the dual-model T-SAE and the single-model T-SAE, was proposed in this paper. The hyperspectral images of oilseed rape leaf and root were acquired under different Pb stress concentrations. The entire region of the oilseed rape leaf (or root) was selected as the region of interest (ROI) to extract the spectral data, and standard normalized variable (SNV), first derivative (1st Der) and second derivative (2nd Der) were used to preprocess the ROI spectra. Besides, the principal component analysis (PCA) algorithm was used to reduce the dimensionality of the spectral data before and after preprocessing. Hence, the best pre-processed data was determined for subsequent research and analysis. Furthermore, the SAE deep learning networks were built based on the oilseed rape leaf data, oilseed rape root data, and the combined data of oilseed rape leaf and root based on the best pre-processed spectral data. Finally, the T-SAE models were obtained through transfer learning of the best SAE deep learning network. The results show that the best preprocessing algorithms of the oilseed rape leaf and root spectra were SNV and 1st Der algorithm, respectively. In addition, the prediction set recognition accuracy of the best T-SAE model of Pb stress gradient in oilseed rape plants was 98.75%. Additionally, the prediction set coefficient of determination of the best T-SAE model of the Pb content in the oilseed rape leaf and root data were 0.9215 and 0.9349, respectively. Therefore, a deep transfer learning method combined with hyperspectral imaging technology can effectively realize the the qualitative and quantitative detection of heavy metal Pb in oilseed rape plants.

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