Detection of KIAA1549/BRAF fusion in Moroccan patients with Pediatric Low-Grade Gliomas

Abstract

BackgroundLow-Grade Gliomas are the most common brain tumor in children. They are characterized by a broad spectrum of very heterogeneous tumor subtypes. Their current definition is mainly based on histological criteria which represents an important limit because these classifications suffer from a lack of precision. Recent advances in genomics allow us to considerably deepen our knowledge of the biology of these tumors in order to enrich their current classification. AimIn this work, we studied the frequency of KIAA1549/BRAF fusion in 89 pLGG tissues of Moroccan patients with Pediatric Low-Grade Gliomas, using PCR-RFLP and Fluorescence In Situ Hybridization and we compared our results with the frequency of KIAA1549/BRAF fusion in 123 pHGG tissues of Moroccan patients with Pediatric High-Grade Gliomas. MethodologyWe studied the frequency of KIAA1549/BRAF fusion in 89 pLGG tissues, using PCR-RFLP and Fluorescence In Situ Hybridization. Data was analyzed by using IBM SPSS software v24. Results32 patients with pLGG had a positive KIAA1549/BRAF fusion, compared to 57 with a negative KIAA1549/BRAF fusion by using PCR-RFLP and Fluorescence In Situ Hybridization. Based on our findings, the frequency of KIAA1549/BRAF fusion in Moroccan patients with Pediatric Low-Grade Gliomas is 36.0% in the pLGGs and there is significant relationship between the presence of KIAA1549/BRAF fusion in pLGGs and pHGGs (P < 0.0001). ConclusionWe hope that the results of this study will contribute to the improvement of the molecular and histopathological classification of Pediatric Low-Grade Gliomas (pLGGs) and bring innovations to patient treatment by establishing new clinical trials and experimental studies, as well as a more precise categorization for epidemiological purposes.