Detection of K- ras Codon 12 Mutation by Polymerase Chain Reaction and Differential Dot-Blot Hybridization in Sodium Saccharin-Treated Human RSa Cell

Abstract

Using polymerase chain reaction and differential dot-blot hybridization, point mutations of K- ras codon 12 were investigated in hypermutable human RSa cells treated with sodium saccharin. Cells carried mutational changes after 6 days of treatment at sodium saccharin concentrations of 15 to 30 mg/ml. Results imply that sodium saccharin is a mutagen which induces genetic mutation and, furthermore, that the assay system applied here will be beneficial in identifying mutagen-induced K- ras oncogene mutations in human cells in vitro.