Detection of Intraspecific Genetic Relationship in Created Ibises Nipponia nippon through Randomly Amplified Polymorphic DNA Analysis.

Abstract

There are seventeen Crested Ibises (Nipponia nippon) in Beijing Zoo. Randomly amplified polymorphic DNA (RAPD) was first used to research intraspecific genetic relationships of eight ibises of them. Twenty arbitrary primers were used to amplify genomic DNA of eight Ibises and seventeen primers have distinct RAPD patterns in each amplification. One hundred and sixty-eight amplified fragments were generated by RAPD and one hundred and two fragments were shared by eight Ibises. Hierachical Cluster analysis of the RAPD data was used to calculate the similarity coefficients by spss/pc+ program. Based on the correlation similarity coefficient matrix, a dendrogram was constructed. The dendrogram indicates the genetic relationship of eight Crested Ibises. This study is the basis of constructing the genetic relationships dendrogram of all individuals, which is helpful in working out a better Crested Ibis conservation plan.