Detection of Insect Immunity Substances (Lectins) in the Midgut Extracts from Larvae and Adult Red Palm Weevil Rhynchophorus ferrugineus (Olivier) in Al-Ahsa, Saudi Arabia

Abstract

The hemagglutination activities of midgut (MG) fractions of red palm weevil, Rhynchophorus ferrugineus against mammalian erythrocytes (RBC) from man (ABO), rabbit, horse and sheep were studied. The highest titers were seen with rabbit RBC (p < 0.05) followed by human group B, human group O, horse, human group A, human group AB and sheep, respectively. Insect age and nutritional status significantly influenced the agglutinating activities. Some of the unfed insects were having low activities (p > 0.01) than the well fed insects (p < 0.05). The agglutination activities were linearly correlated with the insect age. The agglutination activities were higher in old insects than the young insects (p < 0.05). Further studies are needed to investigate the lectins in the hemolymph of the red palm weevil, Rhynchophorus ferrugineus beside demonstrating their agglutination activities against the natural entomopathogenes organisms of this economically important insect for use in its biological control.