Detection of influenza A virus RNA in birds by optimized Real—Time PCR system

Abstract

Objective To evaluate the use of Real-Time PCR system based on specific amplification of matrix protein gene fragment for influenza A virus RNA detection in cloacal swabs from wild birds. Methods Sensitivity, specificity and reproducibility of analysis results were identified. Study of cloacal swabs from wild birds for influenza A virus presence was performed. Results Reproducibility of low concentrations of virus detection in samples by Real-Time PCR was significantly higher than that of detection based on cytopathic effect of viruses grown on MDCK cell culture. Conclusions Real-Time PCR system for influenza A virus RNA detection is developed and applied for virus surveillance study.