Abstract
Polystyrene nanoparticles (PSNPs) in comparison of metallic nanoparticles are more economic, easy to prepare, less toxic and multipurpose in usefulness; also exhibits better attachment towards protein, especially antigens and antibodies. In the present investigation PSNPs were prepared by chemical induced microemulsion polymerization method and were characterized on account of diameter, shape and distribution using Transmission Electron Microscopy (TEM). The chemical structure of prepared nanoparticles was analyzed by Fourier Transform Infrared (FTIR) spectrometer. The resultant nanoparticles were spherical in shape, uniformly distributed and had an average diameter of 75 nm. These well characterized PSNPs were tested for surface modification of Quartz Crystal Microbalance (QM) for the detection of infectious bursal disease virus (IBDV). The detection limit (104 cfu/ml) and the cross reactivity of the tested nanobiosensor was cross checked with non-specific antigen RK2 (specific to Cd-Rhizobacteria, 105 cfu/ml), which established the specificity of device.
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