Abstract
Detection of icaA, icaD, and bap genes and biofilm production in Staphylococcus aureus and non-aureus staphylococci isolated from subclinical and clinical bovine mastitis
Highlights
The aim of the present study was to verify the biofilm production by S. aureus and Non-aureus staphylococci (NAS) isolates derived from subclinical and clinical bovine mastitis cases and evaluate the presence of icaA, icaD, and bap in these isolates by polymerase chain reaction (PCR)
A total of nine monthly visits were performed in order to obtain 100 strains of S. aureus from subclinical mastitis cases and 100 strains of NAS, being 92 from subclinical mastitis cases and eight from clinical mastitis cases
For amplification of icaA and icaD genes, primers and the polymerase chain reaction (PCR) protocol was performed according to Vasudevan et al (2003)
Summary
The aim of the present study was to verify the biofilm production by S. aureus and NAS isolates derived from subclinical and clinical bovine mastitis cases and evaluate the presence of icaA, icaD, and bap in these isolates by PCR. Staphylococcus aureus ATCC 25923 was used as positive control for amplification of the icaA and icaD genes. Qualitative detection of biofilm formation by the isolated strains was evaluated on CRA plates according Freeman et al (1989).
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