Abstract
Staphylococcus aureus is the most important bacterial pathogen associated with mastitis in dairy animals including goats. The production of biofilm by S. aureus is an important virulence factor in the pathogenesis of mastitis. In the study, 70 milk samples were collected from goats affected with clinical mastitis. After preliminary identification, 29 S. aureus isolates were confirmed through 23S rRNA ribotyping. The confirmed S. aureus isolates were further screened for biofilm formation by Congo red agar method and icaA and icaD genes based polymerase chain reaction. On Congo red agar, 25 (86.20%) isolates produced biofilm while 26 (89.65%) and 25 (86.20%) isolates possessed icaA and, icaD genes, respectively. In conclusion, a high prevalence of biofilm producing S. aureus was identified from goat mastitis and possessed adhesion genes.
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