Detection of hypoxanthine based on the electrochemiluminescent of 6-(4-methoxyphenyl)-2-methylimidazo[1,2-a]pyrazin-3(7H)-one on the electrically heated indium–tin-oxide electrode

Abstract

An electrochemiluminescent (ECL) enzyme biosensor was developed for detection of hypoxanthine based on ECL of 6-(4-methoxyphenyl)-2-methylimidazo[1,2-a]pyrazin-3(7H)-one (MCLA). The xanthine oxidase (XOD) was modified on the heated indium–tin-oxide (ITO) electrode, and the ECL enzyme biosensor was found to be electrode temperatures depend, this was because that both the diffusion and convection of compounds near the electrode surface and the catalytic reaction of immobilized enzyme were affected by the temperature of electrode ( T e ). In this work, the highest sensitivity was gained when T e was 36 °C. Under the optimum condition, the linear response range of hypoxanthine (HX) was found to be 0.3–27 μmol/L, and the detection limit (defined as the concentration that could be detected at the signal-to-noise ration of 3) was 0.15 μmol/L.