Abstract
ABSTRACTReactivation of the Polyomavirus BK (BKV) is increasingly recognized as a cause of renal-allograft dysfunction. Currently, patients at risk of nephropathy due to active BKV infection are identified by the presence of cells containing viral inclusion bodies (“decoy cells”) in the urine or by biopsy of the allograft tissue. Demonstrating viral DNA by molecular methods in urine and/or blood is emerging as a non-invasive tool for virology diagnosis and patient management. We report here the development of an in-house polymerase chain reaction (PCR) assay for BKV and its application for detection of BKV DNA in 43/50 (86%) of the urine samples, and in 4/50 (8%) of the peripheral blood leukocytes (PBLs) samples collected from adult kidney transplant recipients between 30 and 70 years of age. Testing for BKV in urine and PBLs from renal-allograft recipients by the use of the PCR is sensitive and specific method for identifying the patients at risk of viral nephropathy.
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