Detection of High-Risk Human Papillomavirus by Chromogenic in Situ Hybridization Method in Liquid-Based Cervicovaginal Cytology Specimens with Atypical Squamous Cells of Undetermined Significance

Melin Özgün Geçer

doi:10.7176/jhmn/80-10

Abstract

Background : Cervical cancer is the second most common cancer after breast cancer in women worldwide. Owing to comprehensive cervical screening programs, a significant decrease has been observed in the incidence of invasive cervical cancers. Human Papillomavirus (HPV) is the underlying etiology in 99.7% of the cases of cervical cancer and is a major risk factor for the development of precancerous and cancerous cervical lesions. Chromogenic in situ hybridization method (CISH) is one of the methods used to detect high risk HPV in liquid -based smears. Aims : In this study, we conducted an archive search in the department of pathology and we used chromogenic in situ hybridization technique to investigate whether HPV DNA was present in liquid -based smears of 68 patients who had been found to have atypical squamous cells of undetermined significance (ASCUS) and whether a cervical intraepithelial lesion was present in the biopsy samples of patients who were positive for HPV DNA. Methods and Material :. We prepared additional samples from liquid -based preparations obtained by the ThinPrep method. The CISH procedure was performed on the Benchmark Automated Slide Stainer (Ventana) according to the manufacturer’s standard protocol. Results and Conclusions : We found HPV DNA in the samples of 10 patients by using INFORM HPV III high risk (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 68, and 70) (Ventana, Tucson, AZ) probe, with CISH method on each additional sample, and biopsy results of the respective patients, indicated low grade squamous intraepithelial lesion (LGSIL) in 8 patients and high grade squamous intraepithelial lesion (HGSIL) in 2 cases. Keywords: Human papillomavirus, chromogenic in situ hybridization, atypical squamous cells of undetermined significance DOI: 10.7176/JHMN/80-10 Publication date: September 30 th 2020

Highlights

Cervical cancer is the second most common cancer type after breast cancer, in women worldwide
Human Papillomavirus (HPV) DNA was tested by chromogenic in situ hybridization method in liquid -based smear specimens of the patients who had been found to have atypical squamous cells of undetermined significance and we have investigated whether a cervical intraepithelial lesion is present in the biopsy specimens of patients who were positive for HPV DNA
A squamous intraepithelial lesion was seen in all biopsy samples taken from the patients who had HPV DNA based on the tests conducted in liquid -based smears

Summary

Introduction

Cervical cancer is the second most common cancer type after breast cancer, in women worldwide. In Turkey, cervical cancer is the third most common cancer after uterine and ovarian cancers according to the data of the Ministry of Health.[1]A significant decrease has been observed in the incidence of invasive cervical cancers, owing to comprehensive cervical cancer screening programs,.[2] Human Papillomavirus Aims: In this study, we conducted an archive search in the department of pathology and we used chromogenic in situ hybridization technique to investigate whether HPV DNA was present in liquid -based smears of 68 patients who had been found to have atypical squamous cells of undetermined significance (ASCUS) and whether a cervical intraepithelial lesion was present in the biopsy samples of patients who were positive for HPV DNA.Methods and Material:.

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