Detection of Hevea brasilensis clones yield potential and susceptibility to tapping panel dryness in Côte d’Ivoire using the 32 and 35 KDa lutoidic proteins

Abstract

This study was carried out to identify protein markers with yield potential and susceptibility to tapping panel dryness (TPD). To achieve this goal, 11 clones, stimulated and non stimulated, yield and susceptibility to TPD were compared. Their lutoid fraction polypeptides were analysed using one and two-dimensional electrophoresis. Susceptibility to TPD appeared as a clonal trait which is not related to yield potential. TPD can occur either in stimulated or non stimulated clones but, overstimulation increase TPD symptoms. Clones PB 235, PB 260 and IRCA 130 are highly susceptible to TPD, whereas IRCA 41, PB 217, AF 261, AVROS 2037 and GT 1 are less susceptible. Productive and less susceptible clones to TPD are characterized by abundant quantity of 35 KDa polypeptide and absence or very small amount of 32 KDa polypeptide. Contrarily, clone susceptible to TPD are characterized by abundant quantity of 32 KDa polypeptide. In unproductive clones (RO 38, TJR 1), 32 KDa protein was more abundant than 35 KDa. Overstimulation induces a decrease of 35 KDa protein intensity. Thus, 32 and 35 KDa polypeptides could be used for identification of Hevea clones yielding potential and susceptibility to TPD. Keywords: Hevea brasiliensis , lutoid, protein, rubber, tapping panel dryness, yield