Abstract
A new detection principle for ligands (here photosystem-II herbicides) based on bacterial luciferase as the detection system is presented. The dependency of this enzyme on long-chain aliphatic aldehydes was harnessed. The requirements for such a system are investigated. Binding properties of artificial aldehydes to the Q B-binding site of photoreaction centres from the phototrophic bacteria Rhodabacter sphaeroides and substrate properties for bacterial luciferase from Photobacterium fischeri of various artificial aldehydes were determined.
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