Abstract
Samples of blood serum obtained from patients with symptoms of acute hepatitis, admitted to infectious disease hospitals of Novosibirsk, Barnaul, and Irkutsk, were investigated. The serologic markers of hepatitis E virus (HEV): IgM and IgG antibodies, were determined by enzyme immunoassay (ELISA). Seropositive samples were investigated by the method of reverse transcription and the polymerase chain reaction (RTPCR). Two variants of HEV were detected, and the first human cases of HEV infection in this region were demonstrated. One of the variants was identified as genotype I, the other as genotype III of HEV. The HEV variants found were cultured in a continuous culture of green monkey kidney cells (4647). The ability of the HEV variant belonging to genotype I to persist in cell culture was shown.
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