Abstract
A radioimmunoassay (RIA) for the measurement of antibody to hepatitis B surface antigen (anti-HB s) in human and non-human sera is described. The principle is based upon our earlier observation that anti-HB s and hepatitis B surface antigen (HB sAg) are soluble in 4.5% w/v polyethylene glycol 4000 but that anti-HB sHB sAg complex precipitated. The use of activated charcoal suspended in polyethylene glycol and dextran solution facilitated the separation of this complex by centrifugation, thus improving reproducibility and sensitivity. The test is approx. 3–5000 times as sensitive as counterelectrophoresis. In a survey of a normal blood donor population, with an incidence of approx. 0.1% positive for HB sAg by RIA, 7–8% were found to have anti-HB s by this method.
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